| Core C - Molecular Biology and Nutritional Biochemistry |
|
|
Discovering Molecular
Mechanisms
The
Molecular Biology & Nutritional Biochemistry Core encourages and facilitates application of molecular biology
and biochemistry tools in nutritional research.
Services Include:
Molecular Biology
|
·
DNA and RNA purification
· Southern Blot
· Northern
Blot
· Western Blot
· RT-
PCR-in situ
hybridization
|
· Probe
preparation
·
PCR & reverse-transcriptase (RT)-
PCR
· Real-time
(quantitative)
PCR
· In situ
hybridization |
Nutritional Biochemistry
|
Oxidative Stress
· Catalase
· Glutathione
peroxidase
· Glutathione
reductase
· Superoxide
dismutase
· Lipid
peroxidation products
|
· Oxidized & reduced glutathione
· Thiobarbituric acid reactive
substances
(TBARS)
· ELISA
· 8-hydroxydiosyguanisine
· Thioredoxin reductase
|
|
Nutrients
· Folate
· Homocysteine
· Vitamin
E
· Vitamin C
· Choline
· Fatty
acids
· Ferritin
· Choline metabolites
· Methionine cycle
intermediates
(SAM, SAH)
|
Inflammation
·Acute-Phase
Proteins
○ C-reactive protein
· Adhesion Molecules
○ E-Selection
○ VCAM-1
○ ICAM-1
· Cytokines and Adiopokines
○ IL-6
○ IL-2
○ TNF |
|
Peptide
Hormones
·
Ghrelin
· Insulin
· C-peptide
· Leptin
· Prolactin |
Other Assays
· Cortisol
· Glucose
·
DHEA
·
Urinalysis
· Virus
isolation
· COMET assay
|
Service Request
If you are interested in requesting services from the Molecular Biology & Nutritional Biochemistry Core, please contact a member of the staff and complete
the online Service Request Form. Someone will be in touch with you shortly regarding this request.
Contact Us:
Qing Shi, Research Specialist
2215
Michael
Hooker
Research
Center
CB # 7461
Chapel Hill,
NC
27599-7461
Phone: (919) 966-7162
Fax: (919) 843-0776
|
|
Last updated May 21, 2008 |
